| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walborn, Mabel R (10)
|
MAUN-5 |
33.40 |
31.66 |
-1.74 |
| 1 |
Giryanskaya, Margarita (10)
|
MANLAC |
|
33.40 |
- |
| 2 |
Hosley, Gabriella G (10)
|
MAUN-6 |
|
34.10 |
- |
| 3 |
Trego, Natalie K (9)
|
MANLAC |
38.62 |
37.62 |
-1.00 |
| 4 |
Fuller, Claire M (10)
|
MANLAC |
40.63 |
39.13 |
-1.50 |
| 5 |
Kraft, Clara R (9)
|
MANLAC |
43.18 |
40.55 |
-2.63 |
| 6 |
Coakley, Sloane V (10)
|
MAUN-6 |
|
41.08 |
- |
| 7 |
Case, Teagan L (10)
|
MANLAC |
|
41.28 |
- |
| 8 |
Huggins, Natalie V (9)
|
MANLAC |
42.85 |
42.62 |
-0.23 |
| 9 |
Masters, Katherine H (10)
|
MANLAC |
|
45.53 |
- |
| 10 |
Mattiacci, Deanna M (10)
|
MAPENN |
|
49.60 |
- |
| 11 |
Prentice, Samantha K (9)
|
MAPENN |
|
59.64 |
- |